I had to repeat my PCR today because the lab said my PCR from 3 wks. ago did not have enough DNA. The lab took the usual three tubes full of blood. I assumed DNA was included in the samples. Is this possible or is the lab shifting blame?
Not enough DNA?
Started by
SunNsand
, Oct 06 2011 01:21 PM
5 replies to this topic
#2
CallMeLucky
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Posted 06 October 2011 - 01:56 PM
Totally guessing, but it sounds like the sample wasn't viable. Not an issue of what they took out, it sounds like it didn't last. Maybe there wasn't enough preservative in the tube or something like that.
Date - Lab - Scale - Drug - Dosage MG - PCR
2010/Jul - MSKCC - Non-IS - Gleevec - 400 - 1.2%
2010/Oct - MSKCC - Non-IS - Gleevec - 400 - 0.25%
2010/Dec - MSKCC - Non-IS - Gleevec - 400 - 0.367%
2011/Mar - MSKCC - Non-IS - Gleevec - 400 - 0.0081%
2011/Jun - MSKCC - Non-IS - Gleevec - 400 - 0%
2011/Sep - MSKCC - Non-IS - Gleevec - 400 - 0.00084%
2011/Dec - MSKCC - Non-IS - Gleevec - 400 - 0%
2012/Mar - MSKCC - Non-IS - Gleevec - 400 - 0.004%
2012/Jun - MSKCC - Non-IS - Gleevec - 400 - 0%
2012/Sep - MSKCC - Non-IS - Gleevec - 400 - 0%
2012/Dec - MSKCC - Non-IS - Sprycel - 100 - 0%
2013/Jan - Quest - IS - Sprycel - 50-60-70 - 0%
2013/Mar - Quest - IS - Sprycel - 60-70 - 0%
2013/Apr - CUMC - Non-IS - Sprycel - 50 - 0.036%
2013/May - CUMC - Non-IS - Sprycel - 50 - 0.046%
2013/Jun - Genoptix - IS - Sprycel - 50 - 0.0239%
2013/Jul - Genoptix - IS - Sprycel - 70 - 0.0192%
2013/Jul - Genoptix - IS - Sprycel - 70 - 0.0034%
2013/Oct - Genoptix - IS - Sprycel - 70 - 0.0054%
2014/Jan - Genoptix - IS - Sprycel - 70 - 0.0093%
2014/Mar - Genoptix - IS - Sprycel - 100 - 0.013%
2014/Apr - Genoptix - IS - Sprycel - 100 - 0.0048%
2014/Jul - Genoptix - IS - Sprycel - 100 - 0%
2014/Nov - Genoptix - IS - Sprycel - 100 - 0.047%
2014/Dec - Genoptix - IS - Sprycel - 100 - 0%
2015/Mar - Genoptix - IS - Sprycel - 100 - 0%
2015/Jun - Genoptix - IS - Sprycel - 100 - 0%
2015/Sep - Genoptix - IS - Sprycel - 100 - 0%
2015/Dec - Genoptix - IS - Sprycel - 100 - 0%
2016/Mar - Genoptix - IS - Sprycel - 100 - 0.0228%
2016/Jun - Genoptix - IS - Sprycel - 100 - 0%
2016/Sep - Genoptix - IS - Sprycel - 100 - 0%
2016/Dec - Genoptix - IS - Sprycel - 100 - 0%
2017/Mar - Genoptix - IS - Sprycel - 100 - 0%
2017/Jun - Genoptix - IS - Sprycel - 100 - 0%
2017/Sep - Genoptix - IS - Sprycel - 100 - 0%
2017/Dec - Genoptix - IS - Sprycel - 100 - 0%
#3
Trey
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Posted 06 October 2011 - 03:51 PM
There is enough DNA in a test tube of blood to verify your identity a million times. Your problem is as Lucky guessed.
The sample was spoiled. The DNA (technically, RNA in this case is needed) was already mostly (or all the way) decomposed, so the PCR could not be done.
#4
PhilB
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Posted 06 October 2011 - 04:55 PM
In the interests of completeness, the other possible explanations are that you are:
- A robot
- An alein
- An alien robot
#5
SunNsand
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Posted 06 October 2011 - 05:45 PM
Lol, thanks for reassuring me I am still human. This was the first time my sample was sent to the new lab they are now using. Looks like good times are ahead for me and future tests and I even went on a Monday like you all say to do. Strike 1 for them!
#6
Marnie
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Posted 06 October 2011 - 10:00 PM
As always, Phil. . .you crack me up.
Just got home from 2 agonizingly long days of teaching and parent teacher conferences. Nothing like being at work from 6:30 a.m. until 8:30 p.m. two days in a row, non-stop with a classroom full of energized middle schoolers and then their parents at night. This on top of being sicker than I've been in a long, long time. Totally flattened for 3 days with the crud. Wish I were an alien robot. . .would have been easier to get thru this week at work. I knew I was sick when the thought of the motorcycle or any alcoholic beverage held absolutely no appeal. I must be on the mend, though, because after getting home tonight at 8:30, I am sitting here with a glass of wine. . .first I've had in awhile.
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