I just got my first pcr test back and don't understand what I'm looking at. Hoping you all can help explain it to me.
bcr/abl, QT PCR, cell-based - 0.006 H 0.000 ratio Q
The ratio of the fusion transcripts to an international control transcript (ABL) is shown.
BCR-ABL:ABL ratio 2.838 Log redustion Q
This assay is a real time quantitative RT-PCR assay. Extracted sample RNA is subjected to a real time RT-PCR reaction to measure quantity of the two types of BCR-ABL fusion transcripts (b2a2/b3a2 and e1a2) .
It goes on to explain to say the analytical sensitivity of the test is 1 tumor cell in 100000 normal cells. The median of BCR-ABL:ABL ratio in previously untreated CML patients is 4.1325 in peripheral blood. New England Journal states its not necessary to know the baseline to calculate the change and this calculation should be based on the above median.
Log reduction is calculated with this formula
log10(4.1325)-log10(bcr-abl:abl ratio) unit log reduction.
Any help would be much appreciated. My fish last month was a 2.2.
Thank you all so much!