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Failed to produce sufficient metaphases for FISH Test ?? what is that

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#1 Nora@CML


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Posted 28 April 2012 - 07:30 AM


As mentioed in above subject. They can not perform FISH test due to the haemotological blood specimen failed to produce sufficient metaphases??

Any idea ??


#2 Trey


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Posted 28 April 2012 - 09:10 AM

There are two types of FISH tests.  One looks for white blood cells (WBCs) in Metaphase and one looks for WBCs in Interphase (which are stages of cell division).  Your test result means there are not enough white blood cells in a certain state of division (metaphase) to do the FISH test.  That is not unusual for patients taking CML drugs when the sample is collected from peripheral blood, so Interphase FISH is used more often on blood samples, and Metaphase FISH can be used on marrow.  Metaphase is generally used on marrow since there are more dividing WBCs found there.  Cells spend a much longer time in Interphase than in Metaphase, so the number of Interphase WBCs is much higher, therefore the test is more likely to find enough usable cells. 


So your Onc should order Interphase FISH instead of Metaphase FISH if the sample is from blood.  If marrow is used, then Metaphase FISH is preferred for its increased accuracy.

#3 Nora@CML


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Posted 28 April 2012 - 12:24 PM

Thank u sooo much Trey

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