Infor from the below link
http://lib.bioinfo.p...horata leukemia
Mei-Chin Lu,
Ying-Chi Du,
Jiunn-Jye Chuu,
Shiuh-Lin Hwang,
Pao-Chuan Hsieh,
Chih-Sheng Hung,
Fang-Rong Chang,
Yang-Chang Wu
Graduate Institute of Natural Products, School of Pharmacy,
Kaohsiung Medical University, Kaohsiung, 807, Taiwan,
Jinx6609@yahoo.com.
The endemic species of Antrodia
camphorate (AC) is a promising chemotherapeutic drug for cancer. We
found that the ethanol extract from wild fruiting bodies of Antrodia camphorata
(EEAC) could induce HL 60 cells apoptosis via histone hypoacetylation,
up-regulation of histone deacetyltransferase 1 (HDAC 1), and
down-regulation of histone acetyltransferase activities including GCN 5,
CBP and PCAF in dose-dependent manner. In combination with histone
deacetylase inhibitor, trichostatin A (TSA), did not block EEAC-induced
apoptosis. Interestingly, combined treatment (100 nM of TSA and 100
mug/ml EEAC) caused synergistic inhibition of cell growth and increase
of apoptotic induction. EEAC could effectively increase the cytotoxic
sensitivity of TSA through the up-regulation of DR5 and NFkappaB
activation. In this present study, bioassay-guided fractionation of EEAC
led to a major active compound, zhankuic acid A, as the bioactive
marker. Moreover, our findings may represent an experimental basis for
developing EEAC as a potential chemotherapeutic adjuvant.
